A method for the assessment of the parameters of an automated system for analysing the microbiological objects motility

Abstract

A large number of biological objects are characterized by their movement. Dependence of the movement direction of individual cells or its line by using of external light, electromagnetic radiation or chemical effects on a solution can be examined by using optoelectronic system for tracks analysing and counting the number of objects. In the case of analysing such objects characteristics, their number in the system field of vision is a random number. The similar problem arises in the biotechnology of reproduction and selection of livestock when creating new breeds with programmed properties and when applying artificial insemination. In the process of creation such systems the problem of determination the probability with which the system can distinguish two or more objects appearing in its field of vision is arisen. In the paper the method of determination the probability depending on the system resolution and objects number in its field of vision is suggested. Since the objects number in the field of vision can be varied or set by changing the solution concentration, the goal of the work is to determine the number of objects when the object merge does not occur with a probability that exceed some predetermined level of significance that is close enough to one.